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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Clearing factor ： ウィキペディア英語版 Clearing factor In centrifugation the clearing factor or k factor represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed. It can be used to estimate the time $t$ (in hours) required for sedimentation of a fraction with a known sedimentation coefficient $s$ (in svedbergs): : $t\; =\; \backslash frac$ The value of the clearing factor depends on the maximum angular velocity $\backslash omega$ of a centrifuge (in rad/s) and the minimum and maximum radius $r$ of the rotor: : $k\; =\; \backslash frac\})\}\; \backslash times\; \backslash frac$ As the rotational speed of a centrifuge is usually specified in RPM, the following formula is often used for convenience:〔("Basics of Centrifugation" )〕 : $k\; =\; \backslash frac\})\}$ Centrifuge manufacturers usually specify the minimum, maximum and average radius of a rotor, as well as the $k$ factor of a centrifuge-rotor combination. For runs with a rotational speed lower than the maximum rotor-speed, the $k$ factor has to be adjusted: : $k\_\}$ Where $T$ is the time to pellet a certain particle in hours. Since $S$ is a constant for a certain particle, this relationship can be used to interconvert between different rotors. $\backslash frac\; =\; \backslash frac$ Where $T\_1$ is the time to pellet in one rotor, and $K\_1$ is the K-factor of that rotor. $K\_2$ is the K-factor of the other rotor, and $T\_2$, the time to pellet in the other rotor, can be calculated. In this manner, one does not need access to the exact rotor cited in a protocol, as long as the K-factor can be calculated. (Many online calculators ) are available to perform the calculations for common rotors. ==References== 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Clearing factor」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.